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1. The mother plants should have botanical name and genetic characteristics of the variety.

2. All explants for tissue culture should be taken from tested and or indexed plants. 3. Genetic fidelity of the variety should be maintained.

4. Coding and tagging of cultures and or plantlets indicating the variety and or strain, date of propagation and other relevant information should be done.

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ADMINISTRATIVE ORDER No. 08

Series of 2012

SUBJECT: REVISED SEED AND FIELD STANDARDS FOR THE PRODUCTION HYBRID,

THERMOSENSITIVE GENETIC MALE STERILE (TGMS) LINE AND POLLEN PARENT

Pursuant to the provisions of the Implementing Rules and Regulations of R.A 7308 otherwise known as the Seed Industry Development Act of 1992, and upon approval of the National Seed Industry Council (NSIC), the following standards for seed certification are hereby adopted as a revision of A.O. No. 4, Series of 2010 to ensure the quality of hybrid (F1) and parental seeds (S or TGMS line and P or Pollen Parent).

1. The general requirements of seed

certification applicable to all crops like hybrid rice (F1) and parental lines (S and P lines) as adopted by the National Seed Quality Control Services shall be followed. succeeding specific requirements shall be complied with:

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a. The production area must have fertile soil and temperature regimes adapted for parental lines (S and/or P), with sufficient irrigation and efficient drainage system, not shaded and has no previous record of serious. incidence of insect pests and diseases.

b. The S line must be produced in areas with relatively low temperature that would meet the critical fertility point of the TGMS or S-line. Production of SxP (F1 hybrid) shall be conducted under warm temperature environment meeting the critical sterility point of the TGMS or S line.

Both SxP production area and control plot must undergo at least four (4) series of field inspections: field establishment (10-20 DAT); tillering (30-50 DAT); flowering

stages (55-75 DAT); and one week before harvest, which shall be conducted by NSQCS personnel, deputized seed inspectors and assisted by PhilRice personnel.

d. The seed grower/producer should prepare and plant the control plot of at least 40 sq. m. with the Sline at 15x15 cm spacing and at one seedling per hill same day as S in SxP seed production field. The control plot follows isolation required of TGMS lines. The plot should be kept free of off-types, weeds, insect pests and diseases. Cultural management should also be similar to S in SxP field. Roguing of off-types should be completed before flowering (55-75 DAT) of the S-line. No more rouging should be done thereafter. At maturity, the seed inspector shall harvest 1,000 plant samples inside the control plot and filled grains, if any, are submitted to the NSQCS for weighing and analysis. The 1,000 plant samples harvested occupy 22.5 sq. m. in the control plot. At 3:10 male to female planting ratio, the female parent occupies 7,692 sq. m. in one (1.0) hectare SxP. At four percent (4%) maximum allowable selfed seeds, harvest from the 1,000 plant samples should not exceed 70.0 grams at 14% moisture content (MC). Calculation assumed no failure in isolation, no off-types in the control plots, and 600 kg per hectare seed yield of SxP. In case seed yield per hectare of SXP is greater or lower than 600 kg. per hectare, the corresponding threshold values shall be used as per attached Table 2. If the allowable selfed seeds harvested in the control plot exceeds the standard of four percent (4%), the SxP fields it represent should be rejected and therefore no longer eligible for certification.

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e.

Field standards recommended planting practices, isolation, and varietal purity must be met eligible for certification. The seed inspector shall certify eligibility based on the guidelines and standards for field inspection of F1 and parental seed production as shown in Table 1 hereof (Field Standards for F1 and Parental Seed Production of TGMS-based Two-Line Hybrids).

3. The following seed standards must be complied with:

a. Estimates of 4% maximum allowable selfed seeds at varying seed yields are shown in Table 2 for reference.

b. Seed harvested must undergo seed testing/analysis and meet

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the standards as shown in Table 3 and Table 4.

4. Intensity of sampling and tagging procedures must be in accordance with A.O. No.16, Series of 2010. Tagging of bags of a seed lot that meets the standards of certification must be done by the deputized seed inspectors.

5. Information on agronomic and varietal characteristics and one (1) kg each of the non-viable seed sample of S and P also one (1) kg. of viable seeds of F1 hybrids shall be supplied by the owner (breeder) to the NSQCS Central Office for reference within one (1) month after registration of the varieties in NSIC.

Non-compliance with the above

Certification tags of hybrid parents and requirements shall be a ground for the F1 seeds are as follows:

Breeder (S,P)

Foundation (S,P) Registered (S-line) Registered (P-line) Certified (F1)

denial of seed certification.

This Order shall take effect fifteen (15) days upon publication in the Official Gazette or in one newspaper of general circulation and shall supersede Administrative Order No. 4 Series of 2010.

Recommending Approval:

Approved:

(PROCESO J. ALCALA

Secretary, Department of Agriculture and Chairman, National Seed Industry Counc..

CLARITO M. BARRON, PhD., CESO IV Executive Director and Vice Chairman

National Seed Industry Council

21 February 2012

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052056-5

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Table 1. Field Standards for F1 and Parental Seed Production of TGMS-based Two-line Hybrids

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111

2.

Pollen Parent (P-line)

Distance from possible contamination (m)

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TGMS (S-line)

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*MFE - male fertile environment (less than or equal to critical fertility point of TGMS line at 15-25 days before flowering) **MSE - male sterile environment (greater than or equal to critical sterility point of TGMS line at 15-25 before flowering)

Tabl

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